Next Generation Sequencing

Next- Generation deoxyribonucleic acid sequencing look back Article By SITA DAHAL Table of contents Table of Contents2 scheme:3 existence:3 Different sequencing Technologies and selective selective informationrmation accumulation:4 SEQUENCING BY discount:4 SEQUENCING BY HYBRIDIZATION AND LIGATION:4 superstar MOLECULE SEQUENCING:5 Assembly of great Volume of Data from NGS:7 Applications:7 password:7 Conclusion:9 dot of generator list:10 Abstract: The topic, DNA sequencing has been at hype from the ending two decades. This review tries to extricate the various facet of this engine room and its wide range of applications non only in biomedical research but too in an opposite(prenominal) areas. at that organize have been rapid technological advancements making most of the lot obsolete. At present, second multiplication sequencers are being replaced by third generation sequencers having dogged orders. The importance of bioinformatics and various other questions like where will much(prenominal) huge amount of info lead us are dealt. We analyze by this subscribe that completion of human genome sequencing was not the end but decent a starting point to personalized medicine.

Genome sequencing has helped to develop new-sprung(prenominal) vaccines and the drastic reduction in terms of sequencing platform has make it contingent to be used as clinical symptomatic means in near future. Introduction: In 1977, Fredrick Sanger demonstrable the jump sequencing technique known as chain margin manner. This procedure utilizes dideoxynucleoside triphosphates (ddTTP) which are labeled with radioactive phosphate. The incorporation of ddTTP (lacks 3 OH group) terminates the templet directed synthesis of the complementary color strand. The strands are denatured, size fractioned on four different lanes and the standpoint sequences are read by auto radiography (Metzker, M.L., 2010). apply this procedure, Bacteriophage ?X 174 was graduation exercise sequenced genome. It is a less erring method and produces long reads of about 700 bases but endorses various drawbacks like...If you expect to get a plentiful essay, order it on our website: Ordercustompaper.com

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